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MSL Gradient Real-Time Machine thermal cycler for sale
Product Name:  Gradient Real-Time Machine thermal cycler
Latest Price: US$8000-20100/Set
Model No.:  MSL-PCR96G(2)
Weight:  Net weight:0.01 Kg
Minimum Order Quantity:  1 Set Set/Sets
Supply Ability:  300 Sets per Year
Payment Terms:  T/T,L/C,D/A,D/P,Western Union,MoneyGram,PayPal

Quick Details

The most advanced peltier-based semiconductor technology.
High-performance universal power supply.
Large 5.7-inch high-definition LCD display.
Graphical user interface in English and Chinese.
Gradient Real-Time Machine thermal cycler

Packaging & Delivery

Packaging detail:Standard export package
Delivery detail:within 7-10 workdays after receipt of payment


Gradient Real-Time Machine thermal cycler

Cheap Gradient PCR Thermal Cyclers


Gradient Real-Time Machine thermal cycler

Products used the most advanced semiconductor technology, with theimport of original power supply and other key core components, effectively improve the overall performance of the product to ensure products’ amplified speed, extend life, and protect the reliability of analysis results in harsh and complex environments Under the experiment.

Advanced Gradient PCR Thermal Cyclers
Best Gradient PCR Thermal Cyclers

Gradient PCR Machine features

Gradient Real-Time Machine thermal cycler

The most advanced peltier-based semiconductor technology.
High-performance universal power supply.
Large 5.7-inch high-definition LCD display.
Graphical user interface in English and Chinese.
Power-down data protection.
Metal shell, solid, practical, beautiful and generous.
Stepless adjustable hot lid.
Lid can be positioned at any angle.
High-sealing reaction zone, to ensure stable and reliable test.
Used Gradient PCR Thermal Cyclers

MSL Gradient PCR Machine technical specifications

Gradient Real-Time Machine thermal cycler

Capacity                                      96×0.2ml , 96×0.2ml+77×0.5ml 384well
Temperature Range                 0~99.9℃
Heating Rate                              6℃/s
Cooling Rate                              4.5℃/s
Uniformity                                    ≤±0.3℃
Accuracy                                      ≤±0.2℃
Display Resolution                     0.1℃
Temperature Control                  Block\Tube
Ramping Rate Adjustale            0.1~4℃
Gradient Uniformity                     ≤±0.3℃
Gradient Accuracy                       ≤±0.2℃
Gradient Temp. Range                30~99.9℃
Gradient Spread                           1~30℃
Hot Lid Temperature                    30~110℃
Hot Lid Height Adjustale             Stepless Adjustable
Number of Programs                    200
Max.No.of Segment                      9
Max.No.of Step                              9
Max.No.of Cycle                            99
Time Increment/Decrement        1 Sec ~ 9 Min 59 Sec
Temp. Increment/Decrement      0.1~9.9℃
Pause Funcation                           Yes
Auto Data Protection                     Yes
Graphical Display                          Yes
Hold at 4℃                                      Forever
Language                                        中文/English
LCD                                                  5.7inch,320×240 pels
Communication                              USB2.0 , RS232
Dimensions                                     392mm×262mm×252mm (L×W×H)
Weight                                               10kg
Power Supply                                    85~264VAC , 50~60Hz , 600 W
New Gradient PCR Thermal Cyclers

Which real-time or digital PCR system is right for you?

To understand why traditional PCR is limited, it is important to understand what happens during the PCR reaction. A basic PCR run can be divided into three phases:

Index period

Accumulated product exactly doubles with each cycle (assuming 100% reaction efficiency). The reaction is highly specific and precise. Exponential amplification occurs because all reagents are freshly available and the kinetics of the reaction drive the reaction in favor of amplicon doubling.

Linear phase (high variability)
As the reaction continues, some reagents are consumed by amplification. The reaction starts to slow down, and the PCR product no longer doubles with each cycle.

Plateau (endpoint: gel detection using conventional methods)
The reaction stops, no more product is produced, and if left long enough, the PCR product will start to degrade. Because each sample has different reaction kinetics, each tube or reaction will plateau at a different time point. These differences can be seen during the plateau. During the plateau period, conventional PCR performs the measurement, also known as endpoint detection.

Variation in plateau measurements with conventional PCR
Three replicate samples had the same amount of DNA at the start of the reaction and different amounts of PCR product during the plateau phase of the reaction (due to changes in reaction kinetics). Therefore, it will be more accurate to make measurements during the exponential period, where replicate samples expand exponentially.

Real-time PCR measures during exponential phase for more accurate quantitation
Real-time PCR focuses on the exponential phase because it provides more precise data for quantification. During the exponential period, the real-time PCR instrument calculates two values. The threshold line is the level of detection at which the fluorescence intensity of the reaction exceeds background. The PCR cycle at which the sample reaches this level is called the cycle threshold (Ct). Ct values are used for downstream quantification or presence/absence detection. By comparing the Ct values of a sample of unknown concentration with a series of standards, the amount of template DNA in an unknown reaction can be accurately determined.

Digital PCR counts individual molecules for absolute quantification
Digital PCR works by splitting the sample into many individual real-time PCR reactions; some of these reactions contain the molecule of interest (positive) and others do not (negative). After PCR analysis, a fraction of the negative results are used to generate absolute results for the exact amount of target molecule in the sample without the need for reference standards or endogenous controls.

MedsinglongPCR system, from kits, instruments, enzymes, master mixes, reagents, optical cartridges, plastic accessories, spare parts to software etc. Medsinglong real-time quantitative PCR system is a fully integrated quantitative PCR amplification, detection and data analysis system. Medsinglong increases productivity with its unique modular and flexible design, intuitive touch screen interface, advanced and easy-to-use reporting system, and monitors more than 120 attributes through built-in online diagnostic system, so that when analytical problems or instrument problems arise find out the reason.



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